Using a mix containing dUTP and UNG brings security to the assay. Indeed any trace of DNA molecule from previous reaction containing dUTP will be removed by the action of the Uracyl-N-Glycosylase (UNG).
Takyon™ 2X Master Mixes for probe assays are available with dUTP/UNG already incorporated.
TakyonTM core kits contain dUTP and UNG.